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Real Time Analyse af metaboliske profil i<em> Ex Vivo</em> Mus Intestinal Crypt organoide Cultures
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JoVE Journal Biology
Real Time Analysis of Metabolic Profile in Ex Vivo Mouse Intestinal Crypt Organoid Cultures

Real Time Analyse af metaboliske profil i<em> Ex Vivo</em> Mus Intestinal Crypt organoide Cultures

DOI:
10.3791/52026-v

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08:53 min

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November 03, 2014

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,
1Department of Medicine,Albert Einstein College of Medicine, 2Department of Cell Biology,Albert Einstein College of Medicine

Chapters

  • 00:05Title
  • 01:42Crypt Isolation and Culture
  • 06:17Extracellular Flux Assay
  • 07:36Results: Metabolism Assay Using Organoids Derived from 8 month old C57BL/6 Mice
  • 08:20Conclusion

Summary

Automatic Translation

Automatic Translation

Små intestinale krypt organoids dyrket ex vivo tilvejebringe en vævskultur, der sammenfatter vækst af krypter afhængige af stamceller og deres niche. Vi har etableret en metode til at analysere den metaboliske profil i realtid i primær mus krypt organoids. Vi fandt organoids opretholde fysiologiske egenskaber, der er defineret ved deres kilde.

Tags

Keywords: Intestinal Crypt OrganoidMouse IntestineMetabolic ProfileOxygen ConsumptionGlycolytic RateATP ProductionRespiratory CapacityReal-time AnalysisCrypt MicroenvironmentIntestinal Epithelial CellsAdult Stem CellsProgenitor CellsPaneth CellsStromal CellsImmune Cells

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