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Using Real-Time Cell Metabolic Flux Analyzer to Monitor Osteoblast Bioenergetics
 

Using Real-Time Cell Metabolic Flux Analyzer to Monitor Osteoblast Bioenergetics

Article DOI: 10.3791/63142-v • 09:43 min • March 1st, 2022
March 1st, 2022 •
1,2, 1,2, 1,2,3
1Vanderbilt Center for Bone Biology, Division of Clinical Pharmacology, Vanderbilt University Medical Center, 2Department of Medicine, Division of Clinical Pharmacology, Vanderbilt University Medical Center, 3Department of Molecular Physiology and Biophysics, Vanderbilt University

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Summary
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Real-time cell metabolic flux assay measures the oxygen consumption rate and extracellular acidification rate, which corresponds to mitochondrial and glycolytic adenosine triphosphate production, using pH and oxygen sensors. The manuscript explains a method to understand the energy status of osteoblasts and the characterization and interpretation of the cellular bioenergetic status.

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Keywords: Real-time Cell Metabolic Flux Analyzer Osteoblast Bioenergetics ATP Production Rate Metabolite Preference XF Calibrant XF Assay Media Oligomycin A Rotenone Antimycin A Cell Culture Microplate Cell Imaging Software XF ATP Rate Assay Template Sensor Cartridge Utility Plate
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