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Real-time Imaging of Plant Cell Surface Dynamics with Variable-angle Epifluorescence Microscopy
 

Real-time Imaging of Plant Cell Surface Dynamics with Variable-angle Epifluorescence Microscopy

Article DOI: 10.3791/53437-v • 06:31 min • December 12th, 2015
December 12th, 2015 •
1
1Graduate School of Frontier Sciences, The University of Tokyo

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Summary
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The goal of this protocol is to demonstrate how to monitor fluorescently-tagged protein dynamics on plant cell surfaces with variable-angle epifluorescence microscopy, showing blinking dots of GFP-tagged PATROL1, a membrane trafficking protein, in the cell cortex of the stomatal complex in Arabidopsis thaliana.

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Keywords: Cell Surface Dynamics Variable-angle Epifluorescence Microscopy VAEM Real-time Imaging Plant Cell Biology Membrane Trafficking Cytoskeletal Rearrangement Fluorescently-labeled Proteins PATROL1 Stomatal Movement Plasma Membrane Arabidopsis Thaliana Kymograph Analysis Time-lapse Imaging
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