A Manual Small Molecule Screen Approaching High-throughput Using Zebrafish Embryos

Journal

Biology

JoVE Journal
Biology

This content is Free Access.

JoVE Journal Biology

A Manual Small Molecule Screen Approaching High-throughput Using Zebrafish Embryos

DOI:

10.3791/52063-v

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07:45 min

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November 08, 2014

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Shahram Jevin Poureetezadi, Eric K. Donahue, Rebecca A. Wingert

¹Department of Biological Sciences,University of Notre Dame

Chapters

00:05Title
01:33Preparation of Zebrafish Mating Chambers and Collection of Embryos
02:41Arraying and Chemically Treating Zebrafish Embryos
04:34Embryo Fixation and Chemical Screening
05:52Results: Zebrafish Embryonic High-throughput Chemical Screen
07:17Conclusion

Summary

Automatic Translation

The zebrafish is an excellent experimental organism to study vertebrate developmental processes and model human disease. Here, we describe a protocol on how to perform a manual high-throughput chemical screen in zebrafish embryos with a whole-mount in situ hybridization (WISH) read-out.

A Manual Small Molecule Screen Approaching High-throughput Using Zebrafish Embryos

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Chapters

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