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En håndbog Small Molecule Screen Nærmer High-throughput Brug zebrafisk embryoner
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Biology
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JoVE Journal Biology
A Manual Small Molecule Screen Approaching High-throughput Using Zebrafish Embryos

En håndbog Small Molecule Screen Nærmer High-throughput Brug zebrafisk embryoner

DOI:
10.3791/52063-v

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07:45 min

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November 08, 2014

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, ,
1Department of Biological Sciences,University of Notre Dame

Chapters

  • 00:05Title
  • 01:33Preparation of Zebrafish Mating Chambers and Collection of Embryos
  • 02:41Arraying and Chemically Treating Zebrafish Embryos
  • 04:34Embryo Fixation and Chemical Screening
  • 05:52Results: Zebrafish Embryonic High-throughput Chemical Screen
  • 07:17Conclusion

Summary

Automatic Translation

Automatic Translation

Zebrafisken er en fremragende eksperimenterende organisme for at studere hvirveldyr udviklingsprocesser og human sygdom model. Her beskriver vi en protokol om, hvordan du udfører en manuel high-throughput kemisk skærm i zebrafisk embryoner med en hel-mount in situ hybridisering (WISH) udlæsning.

Tags

Keywords: ZebrafishChemical GeneticsSmall MoleculesHigh-throughput ScreeningWhole-mount In Situ HybridizationDevelopmental BiologyDisease MechanismsTranslational ResearchTherapeutic Compounds

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