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Immunology and Infection

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JoVE Journal Immunology and Infection
A Novel In vitro Model for Studying the Interactions Between Human Whole Blood and Endothelium
 

A Novel In vitro Model for Studying the Interactions Between Human Whole Blood and Endothelium

Article DOI: 10.3791/52112-v • 06:27 min • November 21st, 2014
November 21st, 2014 •
1, 1, 1
1Department of Immunology, Genetics and Pathology, Uppsala University

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Summary
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The accessibility of reliable models to investigate vascular blood interactions in humans is lacking. We present an in vitro model of cultured primary human endothelial cells combined with human whole blood to investigate cellular interactions both in the blood (ELISA) and the vascular compartment (microscopy).

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Keywords: In Vitro Model Human Whole Blood Endothelium Cardiovascular Pathologies Coagulation Thrombin Antithrombin Complexes Leukocyte Attachment Tumor Necrosis Factor CD16+ Cells
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