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Journal / Immunology and Infection / Characterization of Human Monocyte Subsets by…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis
 

Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis

Article DOI: 10.3791/57941-v • 09:12 min • October 17th, 2018
October 17th, 2018 •
1,2, 1,2, 3, 4, *1,2, *1,2
1Department of Surgery, Vascular Biology Research Centre, Westmead Hospital, 2Westmead Clinical School, Department of Surgery, The University of Sydney, 3Westmead Research Hub, Westmead Institute for Medical Research, 4Institute for Clinical Pathology and Medical Research, Westmead Hospital
* These authors contributed equally

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Summary
Automatic Translation

Here we present a protocol for characterizing monocyte subsets by whole blood flow cytometry. This includes outlining how to gate the subsets and assess their expression of surface markers and giving an example of the assessment of the expression of M1 (inflammatory) and M2 markers (anti-inflammatory).

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Keywords: Monocyte Subsets Flow Cytometry Whole Blood Analysis CD14 CD16 HLA-DR M1 And M2 Markers Gating Red Blood Cell Lysis White Blood Cell Fixation Sample Preparation Flow Cytometry Setup Quality Control
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