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Immunology and Infection

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JoVE Journal Immunology and Infection
Characterization of Human Monocyte-derived Dendritic Cells by Imaging Flow Cytometry
 

Characterization of Human Monocyte-derived Dendritic Cells by Imaging Flow Cytometry: A Comparison between Two Monocyte Isolation Protocols

Article DOI: 10.3791/54296-v • 08:31 min • October 18th, 2016
October 18th, 2016 •
1, 1, 1, 1, 2, 1, 1
1Department of Immunology, Herbert Wertheim College of Medicine, Florida International University, 2Millipore Sigma

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Summary
Automatic Translation

This study compares two different methods of human monocyte isolation for obtaining in vitro dendritic cells (DCs). Monocytes are selected by adherence or negatively enriched by magnetic separation. Monocyte yield and viability along with MDDC viability, proliferation and CD11c/CD14 surface marker expression will be compared between both methods.

Tags

Keywords: Monocyte Dendritic Cell Isolation Characterization Imaging Flow Cytometry Cell Surface Expression CD11c CD14 PBMC Adherence GMCSF IL4
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