/

/

冲红灯光感受器原子力显微镜使用PeakForce定量纳米机械性能映射

JoVE Journal
Engineering

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Engineering

Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping

Please note that all translations are automatically generated. Click here for the English version.

冲红灯光感受器原子力显微镜使用PeakForce定量纳米机械性能映射

DOI:

10.3791/52164-v

•

14:13 min

•

October 24, 2014

•

Marie E. Kroeger, Blaire A. Sorenson, J. Santoro Thomas, Emina A. Stojković, Stefan Tsonchev, Kenneth T. Nicholson

¹Department of Biology,Northeastern Illinois University, ²Department of Chemistry,Northeastern Illinois University

Chapters

00:05Title
02:27Computer and Microscope Setup
03:45Fluid Cell Assembly and Imaging of Mica Surface
08:25Protein Deposition onto the Mica
10:08Imaging Proteins on Mica
12:20Results: AFM Images of Photoreceptor P3
13:14Conclusion

Summary

Automatic Translation

A method for investigating the structure of a protein photoreceptor using atomic force microscopy (AFM) is described in this paper. PeakForce Quantitative Nanomechanical Property Mapping (PF-QNM) reveals intact protein dimers on a mica surface.

Tags

Atomic Force Microscopy AFM PeakForce Quantitative Nanomechanical Property Mapping PF-QNM Red-Light Photoreceptor RpBphP3 P3 Rhodopseudomonas Palustris Biological Macromolecules Soft Materials

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

互溶的测量准备和摩擦力显微镜，反对聚合物刷

调查单分子粘附的原子力谱

亚纳米级分辨率成像与调幅原子力显微镜在液体

用金纳米粒子-嵌入式膜过滤法选择性脱盐放射性碘阴离子的有效方法

使用单点光曲线绘制的地球样外行星的表面图

优化磁力显微镜分辨率和灵敏度，以可视化纳米级磁畴

原子力显微镜悬臂式纳米压痕：空气和流体中纳米级机械性能测量

Active Probe Atomic Force Microscopy with Quattro-Parallel Cantilever Arrays for High-Throughput Large-Scale Sample Inspection

基于生物杂化蚊虫叮咬的原子力显微镜探针的研制

玻璃纤维增强聚合物（GFRP）复合层压板的拉伸性能表征

Read Article