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生产和使用慢病毒的有选择性地转导原少突胶质前体细胞<em>在体外</em>髓鞘测定
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Production and Use of Lentivirus to Selectively Transduce Primary Oligodendrocyte Precursor Cells for In Vitro Myelination Assays
DOI:

10:00 min

January 12, 2015

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Chapters

  • 00:05Title
  • 01:242K7 Lentivirus Production
  • 05:07Transducing OPCs and Seeding for Co-culture
  • 08:07Results: Transformed OPCs Differentiating on DRGs
  • 09:32Conclusion

Summary

Automatic Translation

Here we present protocols that offer a flexible and strategic foundation for virally manipulating oligodendrocyte precursor cells to overexpress proteins of interest in order to specifically interrogate their role in oligodendrocytes via the in vitro model of central nervous system myelination.

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