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Journal
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Biology
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亚麻花香浸改造（<em>亚麻</em>）生成的转基因后代具有高转化率
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Floral-Dip Transformation of Flax (
Linum usitatissimum
) to Generate Transgenic Progenies with a High Transformation Rate
Please note that all translations are automatically generated.
Click here for the English version.
亚麻花香浸改造（<em>亚麻</em>）生成的转基因后代具有高转化率
DOI:
10.3791/52189-v
•
08:49 min
•
December 19, 2014
•
Nasmah K. Bastaki
,
Christopher A. Cullis
1
Department of Biology
,
Case Western Reserve University
Chapters
00:05
Title
01:25
Growing the Plants
02:39
Floral-Dipping
05:22
Selecting Transformants by PCR
07:12
Typical Direct PCR Results
08:05
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里，我们提出了一个协议，通过花卉浸利用
农杆菌
介导的植物转化改造亚麻。该协议是简单的执行，价格便宜，但得到更高的转化率比亚麻改造当前可用的方法。
Tags
Keywords: Floral-dip Transformation
Linum Usitatissimum
Agrobacterium-mediated Transformation
Binary Vector Plasmid
Linum Insertion Sequence (LIS-1)
High Transformation Rate
T1 Seeds
Direct PCR
Positive Transformants
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