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Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays
 

Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays

Article DOI: 10.3791/52203-v • 08:48 min • November 29th, 2014
November 29th, 2014 •
1, 1, 1, 1
1Institute of Biochemistry, Stuttgart University

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Summary
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Peptide arrays synthesized by the SPOT method can be used to analyze the substrate specificity of Protein lysine methyltransferases (PKMTs) and to define the substrate spectrum of PKMTs to understand their biological role. This protocol describes how to synthesize peptide arrays, methylate them with PKMTs, and analyze the results.

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Keywords: Protein Lysine Methyltransferases Lysine Methylation Post-translation Modification Cell Development Disease Substrate Specificity Peptide Array SPOT Peptide Arrays NSD1 H4K44 H1.5K168 H3K36
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