A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:22Isolating Murine Esophagi
- 03:51Isolating Murine and Human Esophageal Stromal Cells
- 06:58Characterizing Murine and Human Esophageal Stromal Cells
- 09:23Results: Morphology, Cytochemistry and Purity of Cultured Esophageal Stromal Cells
- 10:56Conclusion
We present a protocol to generate primary cultures of murine and human esophageal stromal cells with a myofibroblast phenotype. Cultured cells have spindle shaped morphology, express α-SMA and vimentin, and lack epithelial, hematopoietic and endothelial cell surface markers. Characterized stromal cells can be used in functional studies of epithelial-stromal interactions.
