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Electrophoretic Mobility Shift Assay (EMSA) for the Study of RNA-Protein Interactions
 

Electrophoretic Mobility Shift Assay (EMSA) for the Study of RNA-Protein Interactions: The IRE/IRP Example

Article DOI: 10.3791/52230-v • 12:44 min • December 3rd, 2014
December 3rd, 2014 •
1,2, 1,2, 1,2
1Lady Davis Institute for Medical Research, Jewish General Hospital, 2Department of Medicine, McGill University

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Here we present a protocol to analyze RNA/protein interactions. The electrophoretic mobility shift assay (EMSA) is based on the differential migration of RNA/protein complexes and free RNA during native gel electrophoresis. By using a radiolabeled RNA probe, RNA/protein complexes can be visualized by autoradiography.

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Keywords: RNA-protein Interactions Iron Regulatory Proteins IRP1 IRP2 Iron Responsive Elements IRE UTRs EMSA Gel Retardation Bandshift Assay RNA-binding Proteins Protein-RNA Complexes Post-transcriptional Regulation
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