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凝胶电泳迁移分析(EMSA)为RNA,蛋白质相互作用的研究:IRE / IRP示例
JoVE Journal
Biology
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JoVE Journal Biology
Electrophoretic Mobility Shift Assay (EMSA) for the Study of RNA-Protein Interactions: The IRE/IRP Example
DOI:

12:44 min

December 03, 2014

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Chapters

  • 00:05Title
  • 01:28Preparation of Protein Extracts from Cultured Cells
  • 03:05Preparation of Protein Extracts from Mouse Liver and Spleen
  • 04:56Synthesis of Radiolabeled IRE-containing RNA Probe
  • 05:55Purification of Radiolabeled IRE-containing RNA Probe
  • 07:48Electrophoretic Mobility Shift Assay (EMSA)
  • 10:27Results: The Iron-dependence of IRE/IRP Interactions
  • 12:17Conclusion

Summary

Automatic Translation

在这里,我们提出了一个协议分析RNA /蛋白质相互作用。电泳迁移实验(EMSA)是基于RNA的/蛋白复合物和游离RNA在天然凝胶电泳迁移差异上。通过使用放射性标记的RNA探针,RNA /蛋白质复合物可以通过放射自显影进行显现。

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