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מדידה של תפקוד של שריר חלק ברקמות מבודדות אמבט-היישומים למחקר פרמקולוגיה
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Measurement of Smooth Muscle Function in the Isolated Tissue Bath-applications to Pharmacology Research
DOI:

09:05 min

January 19, 2015

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Chapters

  • 00:05Title
  • 01:00Notes on Preparing the System
  • 02:17Preparing the Aortic Rings
  • 04:31Setting-up the Aortic Rings in the Tension Apparatus
  • 06:31Initial Challenge and Experimentation
  • 08:00Results: Scoring the Half-maximum Values for Phenylephrine
  • 08:45Conclusion

Summary

Automatic Translation

This protocol describes the measurement of isometric contraction in an isolated smooth muscle preparation, using an isolated tissue bath system and computer-based data acquisition.

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