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激光捕获显微切割 - 个人多巴胺神经元的分离和整个腹侧被盖区的示范
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
Laser Capture Microdissection – A Demonstration of the Isolation of Individual Dopamine Neurons and the Entire Ventral Tegmental Area
DOI:

08:29 min

February 06, 2015

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Chapters

  • 00:05Title
  • 01:12Preparaion of Slides and Tissue for Laser Capture Microscopy
  • 03:08Laser Capture Microdissection System
  • 05:19Capturing the Ventral Tegmental Area from PEN Membrane Slides
  • 06:19Results: Dopamine Neuron Isolation Using Laser Capture Microdissection
  • 08:05Conclusion

Summary

Automatic Translation

个别的多巴胺神经元的分离或用直接或间接的免疫组织化学的腹侧被盖区,使用激光捕获显微切割证实。对于使用红外激光从玻璃载片隔离的组织,并从用红外和紫外激光的组合膜载玻片参数进行了讨论。

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