JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Medicine
/
聚丙烯酰胺凝胶侵袭伪足和牵引力化验的肿瘤细胞
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Medicine
Polyacrylamide Gels for Invadopodia and Traction Force Assays on Cancer Cells
Please note that all translations are automatically generated.
Click here for the English version.
聚丙烯酰胺凝胶侵袭伪足和牵引力化验的肿瘤细胞
DOI:
10.3791/52343-v
•
08:48 min
•
January 04, 2015
•
Rachel J. Jerrell
,
Aron Parekh
1
Department of Otolaryngology
,
Vanderbilt University Medical Center
Chapters
00:05
Title
01:22
Preparation of Glass Coverslips for Polyacrylamide Gels
02:59
Preparation of Polyacrylamide Gels for Invadopodia Assays
05:09
Preparation of Extracellular Matrix for Invadopodia Assays
07:10
Results: Invadopodia and Traction Force Assays
08:24
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
在肿瘤微机械刚性起着通过增加侵袭伪足活性和肌动球蛋白收缩驱动恶性行为了至关重要的作用。用聚丙烯酰胺凝胶(部分分配),侵袭伪足和牵引力测定可以被用于研究癌细胞的侵入性和收缩特性响应于矩阵刚性。
Tags
Polyacrylamide Gels
Invadopodia
Traction Force
Cancer Cells
Extracellular Matrix (ECM)
Actin
Actomyosin Contractility
Rigidity
Migration
Invasion
Proteolytic Degradation
Contractile Forces
In Vitro Assays
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
加工的主要脑肿瘤组织的干细胞检测和流量排序
人牙髓干细胞的分离,鉴定和比较性恒牙使用两种不同的方法,得出
来自患者的细胞培养和分离的CD133<sup> +</sup>假定的肿瘤干细胞的恶性黑色素瘤
人类前列腺上皮细胞形成的使用鼠类泌尿生殖窦间质细胞和人类干细胞的组织重组
半自动适应循环肿瘤细胞(CTC)分析临床和临床前研究中的应用
在乳腺癌细胞雌激素调节小分子RNA的剖析
癌症干细胞从人类前列腺癌样本隔离
为获取原发性卵巢癌细胞固体样品的方法
丰富的人力细胞系化疗耐药性卵巢癌干细胞
研究胰腺癌干细胞特性开发新的治疗策略
Read Article