Journal
/
/
聚丙烯酰胺凝胶侵袭伪足和牵引力化验的肿瘤细胞
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Polyacrylamide Gels for Invadopodia and Traction Force Assays on Cancer Cells
DOI:

08:48 min

January 04, 2015

,

Chapters

  • 00:05Title
  • 01:22Preparation of Glass Coverslips for Polyacrylamide Gels
  • 02:59Preparation of Polyacrylamide Gels for Invadopodia Assays
  • 05:09Preparation of Extracellular Matrix for Invadopodia Assays
  • 07:10Results: Invadopodia and Traction Force Assays
  • 08:24Conclusion

Summary

Automatic Translation

在肿瘤微机械刚性起着通过增加侵袭伪足活性和肌动球蛋白收缩驱动恶性行为了至关重要的作用。用聚丙烯酰胺凝胶(部分分配),侵袭伪足和牵引力测定可以被用于研究癌细胞的侵入性和收缩特性响应于矩阵刚性。

Related Videos

Read Article