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线粒体的制备及透气性评估，从骨骼肌组织通过穿刺活检获得的分离
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal
Medicine
Preparation and Respirometric Assessment of Mitochondria Isolated from Skeletal Muscle Tissue Obtained by Percutaneous Needle Biopsy
Please note that all translations are automatically generated.
Click here for the English version.
线粒体的制备及透气性评估，从骨骼肌组织通过穿刺活检获得的分离
DOI:
10.3791/52350-v
•
11:27 min
•
February 07, 2015
•
Manish S. Bharadwaj
,
Daniel J. Tyrrell
,
Mary F. Lyles
,
Jamehl L. Demons
,
George W. Rogers
,
Anthony J. A. Molina
1
Department of Internal Medicine, Section on Gerontology and Geriatric Medicine
,
Wake Forest School of Medicine
,
2
Seahorse Biosciences
Chapters
00:05
Title
01:38
Skeletal Muscle Biopsy
03:20
Mitochondrial Isolation and Washing
06:35
Perform Extracellular Flux (XF) Assays
08:32
Results: Respirometric Assessment of Mitochondria
10:11
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
方法活检
股外侧
，准备纯化线粒体和透气性分析描述。使用小肌肉体积使得该技术适用于临床研究应用。
Tags
Mitochondria
Skeletal Muscle
Percutaneous Needle Biopsy
Respirometry
Extracellular Flux Analysis
Electron Transport Chain
COX IV
GAPDH
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