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Elektro och morfologisk karakterisering av neurala Microcircuits i akut hjärnan skivor Använda Kopplade Patch-Clamp Inspelningar
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JoVE Journal Neuroscience
Electrophysiological and Morphological Characterization of Neuronal Microcircuits in Acute Brain Slices Using Paired Patch-Clamp Recordings
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Elektro och morfologisk karakterisering av neurala Microcircuits i akut hjärnan skivor Använda Kopplade Patch-Clamp Inspelningar

DOI:
10.3791/52358-v

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10:24 min

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January 10, 2015

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, ,
1Institute of Neuroscience and Medicine (INM-2),Research Centre Jülich, 2Department of Psychiatry, Psychotherapy and Psychosomatics, Medical Faculty, JARA,RWTH Aachen University

Chapters

  • 00:05Title
  • 02:22Paired Patch-Clamping: Neuronal Connections with a Low Connectivity
  • 05:11Paired Patch-Clamping: Neuronal Connections with a High Connectivity
  • 06:16Neuronal Reconstruction and Synaptic Contact Localization
  • 07:17Results: Morphology and Electrophysiology of Synaptic Connections in Cortical Neuronal Microcircuits
  • 09:35Conclusion

Summary

Automatic Translation

Automatic Translation

Patch-clamp recordings and simultaneous intracellular biocytin filling of synaptically coupled neurons in acute brain slices allow a correlated analysis of their structural and functional properties. The aim of this protocol is to describe the essential technical steps of electrophysiological recording from neuronal microcircuits and their subsequent morphological analysis.

Tags

Keywords: Paired Patch-clamp RecordingAcute Brain SliceNeuronal MicrocircuitsSynaptic TransmissionElectrophysiologyMorphologyConnectivityChemical SynapsesGap JunctionsNeuron ReconstructionDendritic TruncationAxonal Truncation

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