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Journal / Developmental Biology / An Optogenetic Approach for Assessing Formation…
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Developmental Biology

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JoVE Journal Developmental Biology
An Optogenetic Approach for Assessing Formation of Neuronal Connections in a Co-culture System
 

An Optogenetic Approach for Assessing Formation of Neuronal Connections in a Co-culture System

Article DOI: 10.3791/52408-v • 11:22 min • February 17th, 2015
February 17th, 2015 •
*1, *2, *1, 1, 2, 1
1Neuroscience & Behavioral Disorders, Duke-NUS Graduate Medical School, 2Lee Kong Chian School of Medicine, Nanyang Technological University
* These authors contributed equally

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Summary
Automatic Translation

A protocol to generate a co-culture system consisting of neurons derived from induced pluripotent stem cells (iPSCs), primary cortical neurons and astrocytes is described. This co-culture system allows detection of the formation of synaptic contacts and circuits between new, iPSC-derived neurons and pre-existing cortical neurons expressing channelrhodopsin-2.

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Keywords: Optogenetics Neuronal Connections Co-culture System Induced Pluripotent Stem Cells (iPSCs) Neural Progenitor Cells (NPCs) Astrocytes Cortical Neurons Channelrhodopsin-2 (ChR2) Tandem Dimer Tomato (tdTomato) Synaptic Currents Photostimulation
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