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Developmental Biology

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JoVE Journal Developmental Biology
Alginate Encapsulation of Pluripotent Stem Cells Using a Co-axial Nozzle
 

Alginate Encapsulation of Pluripotent Stem Cells Using a Co-axial Nozzle

Article DOI: 10.3791/52835-v • 07:13 min • July 2nd, 2015
July 2nd, 2015 •
1, 1
1Center for International Research on Integrative Biomedical System, Institute of Industrial Science, The University of Tokyo

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Summary
Automatic Translation

We established a method of encapsulating pluripotent stem cells (PS cells) into alginate hydrogel capsules using a co-axial nozzle. This prevents cells from aggregating excessively and limits the shear stress experienced by cells in suspension culture. The technique is applicable to the mass production of PS cells as well as research on stem cell niche.

Tags

Alginate Encapsulation Pluripotent Stem Cells Co-axial Nozzle Suspension Culture Aggregation Shear Stress Capsule Size Alg-Ca Capsule Alg-PLL Coating Stem Cell Niche Mass Production
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