Yasuyuki Sakai Department of Chemical System Engineering University of Tokyo Biography Publications Institution JoVE Articles Yasuyuki Sakai has not added a biography. If you are Yasuyuki Sakai and would like to personalize this page please email our Author Liaison for assistance. Publications Enhanced Hepatic Differentiation of Human IPS Cells Using Gas Permeable Membrane Tissue Engineering. Part A. Aug, 2018 | Pubmed ID: 30141379 Organ/body-on-a-chip Based on Microfluidic Technology for Drug Discovery Drug Metabolism and Pharmacokinetics. Feb, 2018 | Pubmed ID: 29175062 Clinical Value of F-Fluorodeoxyglucose Positron Emission Tomography/Computed Tomography in Upper Tract Urothelial Carcinoma: Impact on Detection of Metastases and Patient Management Urologia Internationalis. Jun, 2015 | Pubmed ID: 26044598 Preparation of Uniform-sized Hemoglobin-albumin Microspheres As Oxygen Carriers by Shirasu Porous Glass Membrane Emulsification Technique Colloids and Surfaces. B, Biointerfaces. Mar, 2015 | Pubmed ID: 25635614 An On-chip Small Intestine-liver Model for Pharmacokinetic Studies Journal of Laboratory Automation. Jun, 2015 | Pubmed ID: 25385717 Risk Stratification for Bladder Recurrence of Upper Urinary Tract Urothelial Carcinoma After Radical Nephroureterectomy BJU International. May, 2015 | Pubmed ID: 24612074 Bimodal Pattern of the Impact of Body Mass Index on Cancer-specific Survival of Upper Urinary Tract Urothelial Carcinoma Patients Anticancer Research. Oct, 2014 | Pubmed ID: 25275074 The Importance of Physiological Oxygen Concentrations in the Sandwich Cultures of Rat Hepatocytes on Gas-permeable Membranes Biotechnology Progress. Nov-Dec, 2014 | Pubmed ID: 25078970 Equivalent Survival and Improved Preservation of Renal Function After Distal Ureterectomy Compared with Nephroureterectomy in Patients with Urothelial Carcinoma of the Distal Ureter: a Propensity Score-matched Multicenter Study International Journal of Urology : Official Journal of the Japanese Urological Association. Nov, 2014 | Pubmed ID: 25041040 Adhesion of Pancreatic Cancer Cells in a Liver-microvasculature Mimicking Coculture Correlates with Their Propensity to Form Liver-specific Metastasis in Vivo BioMed Research International. 2014 | Pubmed ID: 24900957 Electrochemical Properties of Oxygenated Cup-stacked Carbon Nanofiber-modified Electrodes Physical Chemistry Chemical Physics : PCCP. Jun, 2014 | Pubmed ID: 24817367 Proliferation, Morphology, and Pluripotency of Mouse Induced Pluripotent Stem Cells in Three Different Types of Alginate Beads for Mass Production Biotechnology Progress. Jul-Aug, 2014 | Pubmed ID: 24585713 A Novel 3-dimensional Image Analysis System for Case-specific Kidney Anatomy and Surgical Simulation to Facilitate Clampless Partial Nephrectomy Urology. Feb, 2014 | Pubmed ID: 24468517 Image-based Evaluations of Distribution and Cytotoxicity of Irinotecan (CPT-11) in a Multi-compartment Micro-cell Coculture Device Journal of Bioscience and Bioengineering. Jun, 2014 | Pubmed ID: 24374121 Combination of Microwell Structures and Direct Oxygenation Enables Efficient and Size-regulated Aggregate Formation of an Insulin-secreting Pancreatic β-cell Line Biotechnology Progress. Jan-Feb, 2014 | Pubmed ID: 24265060 First Experience of Active Surveillance Before Systemic Target Therapy in Patients with Metastatic Renal Cell Carcinoma Urology. Jul, 2013 | Pubmed ID: 23806397 Biliary Epithelial Cells Play an Essential Role in the Reconstruction of Hepatic Tissue with a Functional Bile Ductular Network Tissue Engineering. Part A. Nov, 2013 | Pubmed ID: 23745513 Importance of a Diffusion-dominant Small Volume to Activate Cell-secreted Soluble Factor Signaling in Embryonic Stem Cell Culture in Microbioreactors: a Mathematical Model Based Study Journal of Bioscience and Bioengineering. Jul, 2013 | Pubmed ID: 23434300 Human Adipose Tissue-derived Mesenchymal Stem Cells Secrete Functional Neprilysin-bound Exosomes Scientific Reports. 2013 | Pubmed ID: 23378928 Induced Pluripotent Stem Cell-derived Hepatocytes As an Alternative to Human Adult Hepatocytes Journal of Stem Cells. 2012 | Pubmed ID: 23550341 Spatiotemporally Controlled Delivery of Soluble Factors for Stem Cell Differentiation Lab on a Chip. Nov, 2012 | Pubmed ID: 22968416 Direct Oxygen Supply with Polydimethylsiloxane (PDMS) Membranes Induces a Spontaneous Organization of Thick Heterogeneous Liver Tissues from Rat Fetal Liver Cells in Vitro Cell Transplantation. 2012 | Pubmed ID: 22793047 Ubiquitin-specific Peptidase 46 (Usp46) Regulates Mouse Immobile Behavior in the Tail Suspension Test Through the GABAergic System PloS One. 2012 | Pubmed ID: 22720038 Induction of Alternative Fate Other Than Default Neuronal Fate of Embryonic Stem Cells in a Membrane-based Two-chambered Microbioreactor by Cell-secreted BMP4 Biomicrofluidics. Mar, 2012 | Pubmed ID: 22662099 Direct Synthesis of Cup-stacked Carbon Nanofiber Microspheres by the Catalytic Pyrolysis of Poly(ethylene Glycol) Langmuir : the ACS Journal of Surfaces and Colloids. Jun, 2012 | Pubmed ID: 22591331 Enhanced Bile Canaliculi Formation Enabling Direct Recovery of Biliary Metabolites of Hepatocytes in 3D Collagen Gel Microcavities Lab on a Chip. Apr, 2012 | Pubmed ID: 22441571 Rapid Aggregation of Heterogeneous Cells and Multiple-sized Microspheres in Methylcellulose Medium Biomaterials. Jun, 2012 | Pubmed ID: 22440047 Engineering of Implantable Liver Tissues Methods in Molecular Biology (Clifton, N.J.). 2012 | Pubmed ID: 22167650 Use of Liposome Encapsulated Hemoglobin As an Oxygen Carrier for Fetal and Adult Rat Liver Cell Culture Journal of Bioscience and Bioengineering. Nov, 2011 | Pubmed ID: 21813323 Bile Canaliculi Formation by Aligning Rat Primary Hepatocytes in a Microfluidic Device Biomicrofluidics. Jun, 2011 | Pubmed ID: 21799718 Integration of a Pump and an Electrical Sensor into a Membrane-based PDMS Microbioreactor for Cell Culture and Drug Testing Biomedical Microdevices. Oct, 2011 | Pubmed ID: 21728068 Kinetic Modelling of in Vitro Cell-based Assays to Characterize Non-specific Bindings and ADME Processes in a Static and a Perfused Fluidic System Toxicology Letters. Sep, 2011 | Pubmed ID: 21723928 Gas-permeable Membranes and Co-culture with Fibroblasts Enable High-density Hepatocyte Culture As Multilayered Liver Tissues Biotechnology Progress. Jul, 2011 | Pubmed ID: 21630487 Establishment of Self-organization System in Rapidly Formed Multicellular Heterospheroids Biomaterials. Sep, 2011 | Pubmed ID: 21620469 Phthalocyanine-based Fluorescence Probes for Detecting Ascorbic Acid: Phthalocyaninatosilicon Covalently Linked to TEMPO Radicals Chemical Communications (Cambridge, England). May, 2011 | Pubmed ID: 21423994 Towards Single Cell Heat Shock Response by Accurate Control on Thermal Confinement with an On-chip Microwire Electrode Lab on a Chip. Apr, 2011 | Pubmed ID: 21394336 Programming an in Vitro DNA Oscillator Using a Molecular Networking Strategy Molecular Systems Biology. Feb, 2011 | Pubmed ID: 21283142 Cytotoxicity Evaluation of Reactive Metabolites Using Rat Liver Homogenate Microsome-encapsulated Alginate Gel Microbeads Journal of Bioscience and Bioengineering. Apr, 2011 | Pubmed ID: 21239224 Microfibrillated Cellulose Sheets Coating Oxygen-permeable PDMS Membranes Induce Rat Hepatocytes 3D Aggregation into Stably-attached 3D Hemispheroids Journal of Biomaterials Science. Polymer Edition. 2011 | Pubmed ID: 20626957 Enhanced Effects of Secreted Soluble Factor Preserve Better Pluripotent State of Embryonic Stem Cell Culture in a Membrane-based Compartmentalized Micro-bioreactor Biomedical Microdevices. Dec, 2010 | Pubmed ID: 20809277 Electrochemical Biosensor for the Detection of H2O2 from Living Cancer Cells Based on ZnO Nanosheets Analytica Chimica Acta. Jun, 2010 | Pubmed ID: 20685417 Transplantation of a Fetal Liver Cell-loaded Hyaluronic Acid Sponge Onto the Mesentery Recovers a Wilson's Disease Model Rat Journal of Biochemistry. Sep, 2010 | Pubmed ID: 20562412 Modulation of Hepatocarcinoma Cell Morphology and Activity by Parylene-C Coating on PDMS PloS One. 2010 | Pubmed ID: 20300511 Spontaneous Formation of Stably-attached and 3D-organized Hepatocyte Aggregates on Oxygen-permeable Polydimethylsiloxane Membranes Having 3D Microstructures Biomedical Microdevices. Jun, 2010 | Pubmed ID: 20174871 Initial Experience of Diffusion-weighted Magnetic Resonance Imaging to Assess Therapeutic Response to Induction Chemoradiotherapy Against Muscle-invasive Bladder Cancer Urology. Feb, 2010 | Pubmed ID: 19914691 Spontaneous Formation of Highly Functional Three-dimensional Multilayer from Human Hepatoma Hep G2 Cells Cultured on an Oxygen-permeable Polydimethylsiloxane Membrane Tissue Engineering. Part C, Methods. Apr, 2010 | Pubmed ID: 19563254 On-chip Single Embryo Coculture with Microporous-membrane-supported Endometrial Cells IEEE Transactions on Nanobioscience. Dec, 2009 | Pubmed ID: 20142147 A Micropatterned Cell Array with an Integrated Oxygen-sensitive Fluorescent Membrane Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology. Nov, 2009 | Pubmed ID: 19862410 Simple Prophylactic Procedure of Inguinal Hernia After Radical Retropubic Prostatectomy: Isolation of the Spermatic Cord International Journal of Urology : Official Journal of the Japanese Urological Association. Oct, 2009 | Pubmed ID: 19811549 Simultaneous Evaluation of Toxicities Using a Mammalian Cell Array Chip Prepared by Photocatalytic Lithography Analytica Chimica Acta. Oct, 2009 | Pubmed ID: 19808118 A Rapid and Simple Evaluation System for Gas Toxicity Using Luminous Bacteria Entrapped by a Polyion Complex Membrane Chemosphere. Nov, 2009 | Pubmed ID: 19716582 Muscarinic Receptor Activation in the Lumbosacral Spinal Cord Ameliorates Bladder Irritation in Rat Cystitis Models BJU International. Nov, 2009 | Pubmed ID: 19466955 An Orbital Shaking Culture of Mammalian Cells in O-shaped Vessels to Produce Uniform Aggregates Ikki Horiguchi1,2, Ikumi Suzuki3, Takashi Morimura3, Yasuyuki Sakai1,4 1Department of Chemical System Engineering, University of Tokyo, 2Department of Biotechnology, Osaka University, 3Biotech Business Unit, Fukoku Co. Ltd, 4Institute of Industrial Science, University of Tokyo JoVE 57922 Bioengineering Alginate Encapsulation of Pluripotent Stem Cells Using a Co-axial Nozzle Ikki Horiguchi1, Yasuyuki Sakai1 1Center for International Research on Integrative Biomedical System, Institute of Industrial Science, The University of Tokyo JoVE 52835 Developmental Biology
An Orbital Shaking Culture of Mammalian Cells in O-shaped Vessels to Produce Uniform Aggregates Ikki Horiguchi1,2, Ikumi Suzuki3, Takashi Morimura3, Yasuyuki Sakai1,4 1Department of Chemical System Engineering, University of Tokyo, 2Department of Biotechnology, Osaka University, 3Biotech Business Unit, Fukoku Co. Ltd, 4Institute of Industrial Science, University of Tokyo JoVE 57922 Bioengineering
Alginate Encapsulation of Pluripotent Stem Cells Using a Co-axial Nozzle Ikki Horiguchi1, Yasuyuki Sakai1 1Center for International Research on Integrative Biomedical System, Institute of Industrial Science, The University of Tokyo JoVE 52835 Developmental Biology