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Functional Reconstitution and Channel Activity Measurements of Purified Wildtype and Mutant CFTR Protein
 

Functional Reconstitution and Channel Activity Measurements of Purified Wildtype and Mutant CFTR Protein

Article DOI: 10.3791/52427-v • 09:59 min • March 9th, 2015
March 9th, 2015 •
1, 1, 1,2,3
1Programme in Molecular Structure and Function, Hospital for Sick Children, 2Department of Biochemistry, University of Toronto, 3Department of Physiology, University of Toronto

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Summary
Automatic Translation

Described here is a rapid and effective procedure for functional reconstitution of purified wild-type and mutant CFTR protein that preserves activity for this chloride channel, which is defective in Cystic Fibrosis. Iodide efflux from reconstituted proteoliposomes mediated by CFTR allows studies of channel activity and the effects of small molecules.

Tags

Keywords: CFTR Cystic Fibrosis Channel Activity Functional Reconstitution Proteoliposomes Flux-based Assay F508del G551D Phosphorylation Nucleotides Potentiators Inhibitors
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