JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Developmental Biology
/
使用小鸡
GFP
神经管移植和碳水化合物染料 DiI 注射,在鸡胚胎内对神经囊细胞和血管进行双重标记
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal
Developmental Biology
Dual Labeling of Neural Crest Cells and Blood Vessels Within Chicken Embryos Using Chick
GFP
Neural Tube Grafting and Carbocyanine Dye DiI Injection
Please note that all translations are automatically generated.
Click here for the English version.
使用小鸡
GFP
神经管移植和碳水化合物染料 DiI 注射,在鸡胚胎内对神经囊细胞和血管进行双重标记
DOI:
10.3791/52514-v
•
09:57 min
•
May 28, 2015
•
Jean-Marie Delalande
2
,
Nikhil Thapar
,
Alan J. Burns
3
1
Birth Defects Research Centre
,
UCL Institute of Child Health
,
2
Blizard Institute, Centre for Digestive Diseases
,
Queen Mary University of London, Barts and The London School of Medicine and Dentistry
,
3
Department of Clinical Genetics
,
Erasmus University Medical Center, Rotterdam
Chapters
00:05
Title
01:28
Preparing the Host Embryo
03:30
Preparing the Donor Graft Tissue
05:05
Grafting the Tissue
06:11
Injecting Dil into Blood Vessels
07:20
Harvest Embryos for Sectioning or Wholemount Examination
08:05
Results: Dual Labeling in Chimeric Chick Embryos
09:19
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
在这里,我们报告神经波峰细胞和血管的双重标签使用小鸡
GFP
神经管内部嫁接结合血管内DII注射。这种实验技术使我们能够同时在器官发生期间可视化和研究NCC衍生(肠)神经系统和血管系统的发展。
Tags
Neural Crest Cells
Blood Vessels
Chicken Embryos
ChickGFP Neural Tube Grafting
Carbocyanine Dye DiI Injection
Nervous System
Vascular System
Enteric Nervous System
Chick Chimeras
Transgenic ChickGFP
Quail-chick Interspecies Grafting
Cell Development
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
人类神经干细胞从外周血造血祖细胞直接诱导
培养人多能性和神经干细胞的基本和临床前研究一个封闭的细胞培养系统
多光子时间推移成像实时可视化发展: 迁移神经嵴细胞在斑马鱼胚胎中的可视化
从外周单核细胞生成诱导神经干细胞,分化至多巴胺能神经元前体,用于移植研究
小鼠原发性颅神经冠细胞的解剖、培养与分析,用于神经冠细胞脱角和迁移研究
利用人类胚胎干细胞的单细胞培养进行高效神经分化
神经冠状原脂肪衍生干细胞的分离、培养和异位诱导,来自围阴脂肪组织
小鼠胚胎干细胞中神经祖细胞和神经元的分化与表征
一种优化的O9-1/水凝胶系统,用于研究神经嵴细胞中的机械信号
Murine Neural Plate Targeting by In Utero Nano-Injection (NEPTUNE) at Embryonic Day 7.5
Read Article