Journal
/
Developmental Biology
/
Delivery of In Vivo Acute Intermittent Hypoxia in Neonatal Rodents to Prime Subventricular Zone-derived Neural Progenitor Cell Cultures
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Delivery of In Vivo Acute Intermittent Hypoxia in Neonatal Rodents to Prime Subventricular Zone-derived Neural Progenitor Cell Cultures

Delivery of In Vivo Acute Intermittent Hypoxia in Neonatal Rodents to Prime Subventricular Zone-derived Neural Progenitor Cell Cultures

DOI:
10.3791/52527-v

•

05:45 min

•

November 02, 2015

•

, , ,
1Department of Physical Therapy,University of Florida

Chapters

  • 00:05Title
  • 01:23Plethysmograph Chamber Set Up and Cycle Time Calibration
  • 02:54Acute Intermittent Hypoxia Administration and Subventricular Zone Stem/Progenitor Cell Culture
  • 04:33Results: Representative Neurosphere Development
  • 05:17Conclusion

Summary

Automatic Translation

Automatic Translation

This article describes the methodology for administering short periods of intermittent hypoxia to postnatal day 1-8 mouse or rat pups. This approach effectively elicits a robust tissue level “priming effect” on cultured neural progenitor cells that are harvested within 30 min of hypoxia exposure.

Tags

Keywords: In VivoAcute Intermittent HypoxiaNeonatal RodentsSubventricular ZoneNeural Progenitor Cell CulturesCell TherapyNeuronal DifferentiationWhole-body PlethysmographyNon-invasiveNon-stressful

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/52527?article_type=v

Direct Induction of Human Neural Stem Cells from Peripheral Blood Hematopoietic Progenitor Cells
check_url/52527?article_type=v

Stem cell-like Xenopus Embryonic Explants to Study Early Neural Developmental Features In Vitro and In Vivo
check_url/52527?article_type=v

Initiating Differentiation in Immortalized Multipotent Otic Progenitor Cells
check_url/52527?article_type=v

Technique to Target Microinjection to the Developing Xenopus Kidney
check_url/52527?article_type=v

Development of an Ethanol-induced Fibrotic Liver Model in Zebrafish to Study Progenitor Cell-mediated Hepatocyte Regeneration
check_url/52527?article_type=v

Using Primary Neurosphere Cultures to Study Primary Cilia
check_url/52527?article_type=v

A Novel Mammary Fat Pad Transplantation Technique to Visualize the Vessel Generation of Vascular Endothelial Stem Cells
check_url/52527?article_type=v

Quantitative Whole-mount Immunofluorescence Analysis of Cardiac Progenitor Populations in Mouse Embryos
check_url/52527?article_type=v

Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands
check_url/52527?article_type=v

Real-time Live-cell Flow Cytometry to Investigate Calcium Influx, Pore Formation, and Phagocytosis by P2X7 Receptors in Adult Neural Progenitor Cells

Read Article