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A Novel Mammary Fat Pad Transplantation Technique to Visualize the Vessel Generation of Vascular Endothelial Stem Cells
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Chapters
- 00:05Title
- 01:26Isolation of VESCs from the Mouse Mammary Gland
- 04:42In Vivo VESC Vessel Formation Assay Using the Mammay Fat Pad
- 08:05Whole Mount Tissue Preparation for Microscopic Observation
- 10:23Results: Isolation of Vascular Endothelial Stem Cells and Vessel Generation of Tranplanted Cells
- 11:27Conclusion
This work demonstrates a novel approach to assess the proliferation, differentiation, and vessel-forming potential of vascular endothelial stem cells (VESCs) through mammary fat pad transplantation followed by whole-mount tissue preparation for microscopic observation. A lineage tracing strategy to investigate the behavior of VESCs in vivo is also presented.
