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Journal / Biology / Voltage and Calcium Dual Channel Optical Mapping…
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Voltage and Calcium Dual Channel Optical Mapping of Cultured HL-1 Atrial Myocyte Monolayer
 

Voltage and Calcium Dual Channel Optical Mapping of Cultured HL-1 Atrial Myocyte Monolayer

Article DOI: 10.3791/52542-v • 08:25 min • March 23rd, 2015
March 23rd, 2015 •
*1, *1, 1, 2, 3, 1
1Department of Cell and Molecular Physiology, Loyola University Chicago, 2Department of Biomedical Engineering, University of Alabama at Birmingham, 3Department of Bioengineering, Clemson University
* These authors contributed equally

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Summary
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This article describes the technique used to perform dual channel optical mapping in cultured HL-1 atrial cell monolayers. This unique protocol allows the simultaneous visualization of both calcium (Ca) and voltage (Vm) activity in the same area for the detailed detection and analysis of electrophysiological properties of culture monolayers.

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Keywords: Optical Mapping HL-1 Cells Action Potentials Calcium Transients Dual Channel Cardiac Physiology Arrhythmia
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