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Методы Характеризуя совместного развития биопленки и Хабитат неоднородности
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Methods for Characterizing the Co-development of Biofilm and Habitat Heterogeneity
DOI:

09:21 min

March 11, 2015

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Chapters

  • 00:05Title
  • 01:28Flow Cell Setup and Inoculation
  • 03:54Characterizing Biofilm Development in Response to Nutrient Gradients Using Confocal Miscroscopy
  • 05:12Characterizing Internal Solute Transport by Injections of Conservative Fluorescent Tracer
  • 06:39Characterizing the Surrounding Flow Field by Tracking Fluorescent Particles
  • 07:37Representative Analysis of Bacterial Interactions in Biofilms Under Glucose Gradients
  • 08:33Conclusion

Summary

Automatic Translation

Biofilms have complex interactions with their surrounding environment. To comprehensively investigate biofilm-environment interactions, we present here a series of methods to create heterogeneous chemical environment for biofilm development, to quantify local flow velocity, and to analyze mass transport in and around biofilm colonies.

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