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Forbedret Genetisk analyse af enkelte Menneske biopartikler Gendannede af forenklet Mikromanipulation fra Forensic 'Touch-DNA' Evidence
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Biology
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JoVE Journal Biology
Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Forbedret Genetisk analyse af enkelte Menneske biopartikler Gendannede af forenklet Mikromanipulation fra Forensic 'Touch-DNA' Evidence

DOI:
10.3791/52612-v

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11:49 min

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March 09, 2015

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, ,
1Forensic Science Graduate Program, Biochemistry Track,University of Central Florida, 2Department of Chemistry,University of Central Florida, 3National Center for Forensic Science,University of Central Florida

Chapters

  • 00:05Title
  • 02:13Collection of Bioparticles from Touched Objects and Surfaces
  • 04:31Optional Staining of Bioparticles to Aid in Visualization
  • 05:33Isolation of Bioparticles of Interest for Analysis
  • 09:05Combined Microvolume Nucleic Acid Isolation and Autosomal STR Profiling
  • 09:51Results: Enhanced Genetic Analysis of Touch DNA Evidence
  • 11:14Conclusion

Summary

Automatic Translation

Automatic Translation

Her beskriver vi en optimeret og effektiv fjernelse strategi for indsamling af bio-partikler i "røre DNA 'prøver, sammen med en forbedret amplifikationsprotokol involverer en et-trins 5 pi mikro-volumen lyse / STR forstærkning, for at tillade genvinding af kort tandemgentagelse (STR) profiler af bio-partikel donor (s).

Tags

Keywords: Forensic DNA AnalysisTouch DNAMicromanipulationSingle-source DNA ProfilesSTR AmplificationGenetic AnalysisBioparticle RecoveryEnhanced TechniquesTrace Biological Material

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