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JoVE Journal Biology
Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence
 

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Article DOI: 10.3791/52612-v • 11:49 min • March 9th, 2015
March 9th, 2015 •
1,2, 3, 1,2,3
1Forensic Science Graduate Program, Biochemistry Track, University of Central Florida, 2Department of Chemistry, University of Central Florida, 3National Center for Forensic Science, University of Central Florida

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Summary
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Here we describe an optimized and efficient removal strategy for the collection of bio-particles present in ‘touch DNA’ samples, together with an enhanced amplification protocol involving a one-step 5 µl micro-volume lysis/STR amplification, to permit the recovery of short tandem repeat (STR) profiles of the bio-particle donor(s).

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Keywords: Forensic DNA Analysis Touch DNA Micromanipulation Single-source DNA Profiles STR Amplification Genetic Analysis Bioparticle Recovery Enhanced Techniques Trace Biological Material
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