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Forbedret Genetic Analysis of enkelt menneske Bioparticles Gjenn av forenklet Mikromanipulasjon fra Forensic 'Touch DNA' Evidence
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Biology
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JoVE Journal Biology
Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Forbedret Genetic Analysis of enkelt menneske Bioparticles Gjenn av forenklet Mikromanipulasjon fra Forensic 'Touch DNA' Evidence

DOI:
10.3791/52612-v

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11:49 min

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March 09, 2015

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, ,
1Forensic Science Graduate Program, Biochemistry Track,University of Central Florida, 2Department of Chemistry,University of Central Florida, 3National Center for Forensic Science,University of Central Florida

Chapters

  • 00:05Title
  • 02:13Collection of Bioparticles from Touched Objects and Surfaces
  • 04:31Optional Staining of Bioparticles to Aid in Visualization
  • 05:33Isolation of Bioparticles of Interest for Analysis
  • 09:05Combined Microvolume Nucleic Acid Isolation and Autosomal STR Profiling
  • 09:51Results: Enhanced Genetic Analysis of Touch DNA Evidence
  • 11:14Conclusion

Summary

Automatic Translation

Automatic Translation

Her beskriver vi en optimalisert og effektiv fjerning strategi for innsamling av bio-partikler som finnes i 'berøre DNA' prøvene, sammen med en forbedret amplifikasjonsprotokoll involverer en ett-trinns 5 mL mikro-volum lyse / STR forsterkning, for å tillate utvinning av korte tandem gjentagelse (STR) profiler av bio-partikkel-donor (e).

Tags

Keywords: Forensic DNA AnalysisTouch DNAMicromanipulationSingle-source DNA ProfilesSTR AmplificationGenetic AnalysisBioparticle RecoveryEnhanced TechniquesTrace Biological Material

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