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培養細胞および動物組織中のDNAの酸化バイオマーカー、8-オキソ-7,8-ジヒドロ-2'-デオキシグアノシンのHPLC測定、
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JoVE Journal
Chemistry
HPLC Measurement of the DNA Oxidation Biomarker, 8-oxo-7,8-dihydro-2’-deoxyguanosine, in Cultured Cells and Animal Tissues
Please note that all translations are automatically generated.
Click here for the English version.
培養細胞および動物組織中のDNAの酸化バイオマーカー、8-オキソ-7,8-ジヒドロ-2'-デオキシグアノシンのHPLC測定、
DOI:
10.3791/52697-v
•
14:12 min
•
August 01, 2015
•
Nikolai L. Chepelev
,
Dean A. Kennedy
,
Remi Gagné
,
Taryn White
,
Alexandra S. Long
,
Carole L. Yauk
,
Paul A. White
1
Environmental Health Science and Research Bureau
,
Health Canada
Chapters
00:05
Title
02:28
DNA Extraction, Precipitation and Wash
06:06
Enzymatic Digestion
07:44
Preparation of Standards, HPLC Run, and Quantification
11:19
Results: Optimization of 8-oxo-dG Detection and Quantitation
13:10
Conclusion
Summary
Automatic Translation
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Automatic Translation
このプロトコルの目的は、培養細胞または動物組織からのDNAにおけるHPLC-EDによるDNA酸化のマーカー、8-オキソ-7,8-ジヒドロ-2'-デオキシグアノシン(8-オキソdGuo)の検出です。
Tags
Keywords: 8-oxo-dGuo
8-OH-dGuo
8-oxo-7
8-dihydro-2′-deoxyguanosine
DNA Oxidation
Oxidative Stress
HPLC-ED
A549 Cells
KBrO3
Dibenzo(def
p)chrysene
DBC
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