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Utilization of the Soft Agar Colony Formation Assay to Identify Inhibitors of Tumorigenicity in Breast Cancer Cells
 

Utilization of the Soft Agar Colony Formation Assay to Identify Inhibitors of Tumorigenicity in Breast Cancer Cells

Article DOI: 10.3791/52727-v • 11:06 min • May 20th, 2015
May 20th, 2015 •
1, 2, 3, 3, 1
1Department of Baker Institute for Animal Health, Cornell University, 2Department of Molecular Biology and Genetics, Cornell University, 3Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School

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Summary
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Here, we document the use of the soft agar colony formation assay to test the effects of a peptidylarginine deiminase (PADI) enzyme inhibitor, BB-Cl-amidine, on breast cancer tumorigenicity in vitro.

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Keywords: Soft Agar Colony Formation Assay Tumor Progression 3D Cellular Environment Cell Proliferation Cell Migration Cell Transformation Genetic Perturbations PADI2 Breast Cancer BB-Cl-amidine Tumorigenicity MCF10DCIS Cells
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