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Application of the DNA-Specific Stain Methyl Green in the Fluorescent Labeling of Embryos
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JoVE Journal Developmental Biology
Application of the DNA-Specific Stain Methyl Green in the Fluorescent Labeling of Embryos

Application of the DNA-Specific Stain Methyl Green in the Fluorescent Labeling of Embryos

DOI:
10.3791/52769-v

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05:59 min

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May 02, 2015

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, , ,
1Institut Pasteur de Montevideo, 2Sección Biología Celular, Facultad de Ciencias,Universidad de la República

Chapters

  • 00:00Title
  • 01:34Dye Preparation
  • 02:36Whole-mount Embryo Labeling
  • 03:39Confocal Imaging
  • 04:19Representative Results
  • 05:15Conclusion

Summary

Automatic Translation

Automatic Translation

A method for fluorescent staining of fixed biological material with the specific DNA label methyl green is described. Methyl green is used in a diluted aqueous solution and is very resistant to photobleaching. Its far-red emission allows for deep specimen imaging, making it particularly adequate for whole embryos.

Tags

Keywords: DNAMethyl GreenFluorescent LabelingEmbryosZebrafishChickNuclear StainConfocal ImagingPhotobleachingHistological StainDNA-specific

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