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HeLa Based Cell Free Expression Systems for Expression of Plasmodium Rhoptry Proteins
 

HeLa Based Cell Free Expression Systems for Expression of Plasmodium Rhoptry Proteins

Article DOI: 10.3791/52772-v • 09:03 min • June 10th, 2015
June 10th, 2015 •
1, 1
1Department of Biological, Geological, and Environmental Sciences, Cleveland State University

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Expression of malarial proteins in cell based systems remains challenging. We demonstrate two step and one step IVT (in vitro translation) cell free expression systems for expressing malarial recombinant rhoptry proteins from HeLa cells. We use a Ni-resin affinity based purification system to purify the rhoptry proteins.

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Keywords: HeLa Cell-free Expression Plasmodium Proteins Malaria Vaccine Candidates Cell-free Expression Systems Protein Expression In Vitro Human Cell-free Expression PF3D7 0114100 PF3D7 0925900 PF3D7 1361800 Protein Yield Affinity Purification
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