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Medição de Extracelular Ion Fluxos Usando a técnica microeletrodos de auto-referência íon-seletivo
JoVE Journal
Biology
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JoVE Journal Biology
Measurement of Extracellular Ion Fluxes Using the Ion-selective Self-referencing Microelectrode Technique
DOI:

09:18 min

May 03, 2015

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Chapters

  • 00:05Title
  • 01:29Ion-selective Self-referencing Microelectrode Calibration
  • 04:16Ion Flux Measurements
  • 07:38Results: Evidence of Potassium Efflux During Single Cell Wound-healing
  • 08:46Conclusion

Summary

Automatic Translation

Transporters in cell membranes allow differential segregation of ions across cell membranes or cell layers and play crucial roles during tissue physiology, repair and pathology. We describe the ion-selective self-referencing microelectrode that allows the measurement of specific ion fluxes at single cells and tissues in vivo.

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