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JoVE Journal
Developmental Biology

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Neural Differentiation of Mouse Embryonic Stem Cells in Serum-free Monolayer Culture
 

Neural Differentiation of Mouse Embryonic Stem Cells in Serum-free Monolayer Culture

Article DOI: 10.3791/52823-v 06:09 min May 14th, 2015
May 14th, 2015

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Summary

This protocol describes in detail the method for generating neural progenitors from embryonic stem cells using a serum-free monolayer method. These progenitors can be used to derive mature neural cell types or to study the process of neural specification and is amenable to multiwell format scaling for compound screening.

Tags

Neural Differentiation Mouse Embryonic Stem Cells Serum-free Monolayer Culture Neural Progenitors Neural Specification Neurons Glia Reporter Lines Cell Culture
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