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Chapters
- 00:05Title
- 01:37Software Download & Installation and Preparation of Alignment
- 02:52Launching and Using StickWRLD
- 04:37Finding, Selecting, and Saving Interpositional Dependencies (IPDs)
- 05:52Results: StickWRLD Visualization of the Adenylate Kinase Lid Domain Protein
- 06:41Conclusion
Synthetic protein sequences based on consensus motifs typically ignore co-evolving residues, that imply interpositional dependencies (IPDs). IPDs can be essential to activity, and designs that disregard them may result in suboptimal results. This protocol uses StickWRLD to identify IPDs and help inform rational protein design, resulting in more efficient results.
