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海马神经元活动的可视化免疫组化空间后学习神经发育障碍的小鼠模型
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Neuroscience
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JoVE Journal Neuroscience
Immunohistochemical Visualization of Hippocampal Neuron Activity After Spatial Learning in a Mouse Model of Neurodevelopmental Disorders
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海马神经元活动的可视化免疫组化空间后学习神经发育障碍的小鼠模型

DOI:
10.3791/52919-v

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07:43 min

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May 12, 2015

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, , , ,
1Centre for Integrative Biology (CIBIO),University of Trento, 2CNR Neuroscience Institute, Pisa, Italy

Chapters

  • 00:05Title
  • 01:30Preparation of Brain Sections
  • 02:16pERK Immunohistochemistry
  • 05:22Bright-field Imaging of Neuron Activation
  • 05:43Results: pERK is a Reliable Molecular Readout of Learning Dependent Neuronal Activation
  • 06:45Conclusion

Summary

Automatic Translation

Automatic Translation

我们描述了一个协议，免疫组织化学研究海马神经元激活的轮廓暴露于空间学习任务为特征的神经发育起源的认知缺陷小鼠模型。此协议可应用到特征的认知缺陷都遗传或药理学小鼠模型。

Tags

ImmunohistochemistryPERKHippocampusSpatial LearningMorris Water MazeEngrailed-2 KnockoutAutism Spectrum DisordersNeurodevelopmental DisordersNeuronal Activation

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