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Chapters
- 00:05Title
- 02:07Synthesis of a Glycomonomer: 2-Lactobionamidoethyl Methacrylamide (LAEMA)
- 04:26Synthesis of a Glycopolymer Using Reversible Addition-fragmentation Chain-transfer (Raft) Based Polymerization
- 06:38Fluorescent Labeling of the Glycopolymer
- 07:46Assay to Determine the Binding of Glycopolymer to Lectin-coated Beads
- 09:12Results: Characteristics of Glycopolymers Produced by the RAFT Methodology
- 09:59Conclusion
An efficient, three-step synthesis of RAFT-based fluorescent glycopolymers, consisting of glycomonomer preparation, copolymerization, and post-modification, is demonstrated. This protocol can be used to prepare RAFT-based statistical glycopolymers with desired structures.
