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Facile and Efficient Preparation of Tri-component Fluorescent Glycopolymers via RAFT-controlled Polymerization
 

Facile and Efficient Preparation of Tri-component Fluorescent Glycopolymers via RAFT-controlled Polymerization

Article DOI: 10.3791/52922-v • 10:54 min • June 19th, 2015
June 19th, 2015 •
1, 1, 2, 3, 1, 1,4
1Department of Biochemistry, University of Missouri, 2Department of Biological Sciences, University of Missouri, 3Department of Molecular Microbiology & Immunology, University of Missouri, 4Department of Child Health, University of Missouri

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Summary
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An efficient, three-step synthesis of RAFT-based fluorescent glycopolymers, consisting of glycomonomer preparation, copolymerization, and post-modification, is demonstrated. This protocol can be used to prepare RAFT-based statistical glycopolymers with desired structures.

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Keywords: Fluorescent Glycopolymers RAFT Polymerization Glycomonomers 2-lactobionamidoethyl Methacrylamide 2-gluconamidoethyl Methacrylamide N-(2-hydroxyethyl) Acrylamide (4-cyanopentanoic Acid)-4-dithiobenzoate Carboxyfluorescein Succinimidyl Ester Lectin-binding Glycoepitope
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