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Chapters
- 00:05Title
- 01:32Preparation of Peptide Standards
- 02:36LC-SRM Assay Development
- 04:02LC-SRM Assay of Biological Samples
- 07:39Results: LC-SRM Analysis
- 08:37Conclusion
This protocol describes how to perform absolute quantification assays of target proteins within complex biological samples using selected reaction monitoring. It was used to accurately quantify proteins of the mouse macrophage chemotaxis signaling pathway. Target peptide selection, assay development, and qualitative and quantitative assays are described in detail.
