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Isolierung und Kultur Expansion von tumorspezifischen Endothelzellen
JoVE Journal
Medicine
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JoVE Journal Medicine
Isolation and Culture Expansion of Tumor-specific Endothelial Cells
DOI:

10:15 min

October 14, 2015

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Chapters

  • 00:05Title
  • 01:33Isolating Tumors from Mice
  • 02:28Tumor Disscociation and Immunomagnetic Separation
  • 06:46Endothelial Cell (EC) Colony Selection Using Rings
  • 08:31Results: Isolation of Pure Tumor Endothelial Cell Populations
  • 09:47Conclusion

Summary

Automatic Translation

We report a reliable method to isolate and culture primary tumor-specific endothelial cells from genetically engineered mouse models.

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