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JoVE Journal Bioengineering
In Situ Mapping of the Mechanical Properties of Biofilms by Particle-tracking Microrheology
 

In Situ Mapping of the Mechanical Properties of Biofilms by Particle-tracking Microrheology

Article DOI: 10.3791/53093-v • 12:58 min • December 4th, 2015
December 4th, 2015 •
1,2, 2,3,4,5, 2,3,4,6, 2,3
1Interdisciplinary Graduate School, Nanyang Technological University, 2Singapore Centre on Environmental Life Sciences Engineering, Nanyang Technological University, 3School of Biological Sciences, Nanyang Technological University, 4Centre for Marine Bio-Innovation, University of New South Wales, 5School of Biological, Earth and Environmental Sciences, University of New South Wales, 6School of Biotechnology and Biomolecular Sciences Sciences, University of New South Wales

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Summary
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Particle-tracking microrheology investigates the viscoelasticity of materials. Here, the technique is used to determine the viscoelasticity, creep compliance and effective crosslinking roles of different matrix components of a bacterial biofilm. The matrix consists of polymeric substances secreted by the bacteria and its components determine biofilm structure and mechanical properties.

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Keywords: Biofilm Extracellular Polymeric Substances (EPS) Particle-tracking Microrheology Viscoelasticity Crosslinking Mechanical Properties Rheology Shear Stress Bacterial Cells Matrix Components Biofilm Development
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