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miRNA Expression Analyses in Prostate Cancer Clinical Tissues
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JoVE Journal Medicine
miRNA Expression Analyses in Prostate Cancer Clinical Tissues

miRNA Expression Analyses in Prostate Cancer Clinical Tissues

DOI:
10.3791/53123-v

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11:29 min

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September 08, 2015

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, , , , , , , , , ,
1Department of Urology, Veterans Affairs Medical Center, San Francisco,University of California San Francisco

Chapters

  • 00:05Title
  • 02:36Laser Capture Microdissection for Isolation of miRNA
  • 03:59miRNA Preparation from Laser Capture Mirodissection Tissue
  • 04:58miRNA Expression Analysis by In Situ Hybridization
  • 09:00Results: miRNA Expression in Metastatic Cancer Tissues
  • 10:06Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe a simplified protocol for microRNA (miRNA) expression analyses in archived Formalin-Fixed, Paraffin-Embedded (FFPE) or fresh frozen prostate cancer (PCa) clinical tissues employing quantitative real-time PCR (RT-PCR) and in situ hybridization (ISH).

Tags

Keywords: MiRNAProstate CancerBiomarkersFFPERT-PCRIn Situ HybridizationLNA Probes

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