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Chapters
- 00:05Title
- 01:24Generating K14-creER+ R26-SmoM2YFP+ Mice and Inducing SmoM2 Expression in Skin Keratinocytes
- 02:35Skin Specimen Preparation and Epidermis Isolation
- 03:11Single Cell Generation
- 03:54Cell Labeling and Flow Cytometric Analysis
- 05:08Results: Induction of SmoM2 Expression and Skin Keratinocytes
- 06:31Conclusion
Carcinogenesis is a process involving interactions between cancer cells and the cancer microenvironment. To dissect the molecular events, one needs to isolate different cell populations at different stages during cancer development. Using a mouse model for basal cell carcinoma, we describe a protocol for cell analyses during carcinogenesis.
