Journal
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在皮肤癌变细胞群分析
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal Medicine
Cell Population Analyses During Skin Carcinogenesis
DOI:

06:53 min

August 21, 2013

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Chapters

  • 00:05Title
  • 01:24Generating K14-creER+ R26-SmoM2YFP+ Mice and Inducing SmoM2 Expression in Skin Keratinocytes
  • 02:35Skin Specimen Preparation and Epidermis Isolation
  • 03:11Single Cell Generation
  • 03:54Cell Labeling and Flow Cytometric Analysis
  • 05:08Results: Induction of SmoM2 Expression and Skin Keratinocytes
  • 06:31Conclusion

Summary

Automatic Translation

癌变是一个涉及癌细胞和肿瘤微环境之间的相互作用的方法。要解剖的分子事件,一个需要,隔离癌症的发展过程中的不同阶段的不同的细胞群。利用小鼠模型基底细胞癌癌变过程中,我们描述了一个协议,用于细胞分析。

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