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在生理体外模型和病理血流应用的血管细胞重塑调查

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

In Vitro Model of Physiological and Pathological Blood Flow with Application to Investigations of Vascular Cell Remodeling

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在生理体外模型和病理血流应用的血管细胞重塑调查

DOI:

10.3791/53224-v

•

07:30 min

•

November 03, 2015

•

Winston Elliott, Devon Scott-Drechsel, Wei Tan

¹Department of Mechanical Engineering,University of Colorado at Boulder, ²Product Development,Covidien, Ltd.

Chapters

00:05Title
01:04Silanization and Biomolecule Functionalization of Glass Slide
03:25Determination of Pulsatility Index
04:39Flow Chamber Assembly
05:56Results: Cell Morphology and Protein Expression Changes with Flow Conditions
06:48Conclusion

Summary

Automatic Translation

该协议将复制的生理或病理血流体外在确定疾病的病理细胞应答提供帮助。通过引入压力衰减室血液泵的下游，穿过脉管的血流，可以概括和强加于血管内皮或模拟物共培养的单层。

Tags

In Vitro Model Physiological Blood Flow Pathological Blood Flow Vascular Cell Remodeling Vascular Disease Flow Dynamics Pulsatility Index Endothelial Cells Smooth Muscle Cells Fibroblasts Mechano-transduction Cytokine Release Co-culture Cardiac Output Arterial Wall Compliance Pressure Damping

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