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Bioengineering

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JoVE Journal Bioengineering
A Rapid and Quantitative Fluorimetric Method for Protein-Targeting Small Molecule Drug Screening
 

A Rapid and Quantitative Fluorimetric Method for Protein-Targeting Small Molecule Drug Screening

Article DOI: 10.3791/53261-v • 08:34 min • October 16th, 2015
October 16th, 2015 •
1, 1, 2, 1, 1
1Institute of Materials Research and Engineering, Agency for Science, Technology and Research (A*STAR), 2School of Chemical & Biomedical Engineering, Nanyang Technological University

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Summary
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A protocol for small molecular drug screening based on in-situ synthesis of ultrasmall fluorescent gold nanoclusters (Au NCs) using drug-loaded protein as template is presented. This method is simple to determine the binding affinity of drugs to a target protein by a visible fluorescent signal emitted from the protein-templated Au NCs.

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Keywords: Fluorimetric Method Protein-targeting Small Molecule Drug Screening Fluorescent Gold Nanoclusters Albumin Proteins Drug-protein Binding Affinity Drug-protein Binding Constant
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